

This suggests that PPB can interact with the cathode to extract electrons for further CO 2 re-fixation (coming from the Krebs cycle) into the Calvin cycle, thereby improving the C usage. Ag/AgCl) under bio-electrochemical conditions produced comparable amounts of bio-hydrogen but significantly negligible traces of CO 2 as compared to the biological system (11.8 mLCO 2/L). Under bio-electrochemical conditions using a H-cell bio-electrochemical device, the PPB were grown planktonic in the bio-cathode chamber with the optimum substrate ratio of malic acid and Na-glutamate. Under these optimum conditions, a significant fixation of nitrogen in form of single-cell proteins (874.4 mg/L) was also detected. The maximum hydrogen production (H 2_max, 423 mLH 2/L) and production rate (H 2_rate, 2.71 mLH 2/Lh) were achieved using malic acid and Na-glutamate at a COD:N ratio of 100:15. Results suggested that the efficiency of PPB to produce biogenic H 2 was highly dependent on the substrates used. In order to evaluate the hydrogen production under electrode-free conditions, batch experiments were conducted using different nitrogen (NH 4Cl, Na-glutamate, N 2 gas) and carbon sources (malic-, butyric-, acetic- acids) under various COD:N ratios. The aim of this study was to promote bio-hydrogen production and carbon fixation using a mixed culture of purple phototrophic bacteria (PPB) that use infrared radiation in presence or absence of an electrode as electron donor. 3Department of Chemical Engineering, University of Alcalá, Alcalá de Henares, Spainĭomestic and industrial wastewaters contain organic substrates and nutrients that can be recovered instead of being dissipated by emerging efficient technologies.

2IMDEA Water, Parque Tecnológico de Alcalá, Alcalá de Henares, Spain.1Department of Chemical and Environmental Technology, ESCET, Rey Juan Carlos University, Móstoles, Spain.Melero 1, Fernando Martinez 1, Abraham Esteve-Nuñez 2,3 * and Daniel Puyol 1 * Vasiliadou 1, Antonio Berná 2, Carlos Manchon 3, Juan A.
